Gel Analysis FAQs


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Frequently Asked Questions | Gel Analysis

Q. What is the spatial resolution of the UN‑SCAN‑IT gel data?
A. UN‑SCAN‑IT gel operates at full image resolution, and is not limited to screen resolution. Generally 100 to 300 dpi 8-bit grayscale images are sufficient for obtaining good results.

Q. How long does it take to analyze a gel?
A.The time required depends largely upon the quality and complexity of the original image. A clean gel can usually be analyzed in just a few minutes; while "smiles", "smudges", etc. will require additional interaction.

Q. Will UN‑SCAN‑IT gel work with Western blots, Northern blots, Coomassie Gels, TLC, etc.?
A. Yes. It doesn't matter what technique was used to obtain the gel image as long as the original gel, film, or a photo of the gel can be scanned into the computer to generate a standard image file. If the gel is only visible under UV light, generally it is often easiest to take a photo of the gel and scan the photo, or use a digital camera to generate an image.

Q. How can films be scanned?
A. The best results for scanning a film can be obtained by using a scanner that has a transparency mode. This type of scanner shines light through the film (transparency mode) rather than the standard mode which reflects off the film (reflective mode).

Q. Will UN‑SCAN‑IT gel work with wet gels?
A. Yes. As long as you can scan the gel or take a digital photo, then UN‑SCAN‑IT gel can quantify it.

Q. Can UN‑SCAN‑IT gel analyze TLC plates?
A. Yes. The bands in TLC plates can be treated in much the same manner as the bands in a gel. If the bands are only visible under UV light, simply take a photo of the TLC plate and scan the photo, or use a digital camera to generate an image.

Q. Does UN‑SCAN‑IT gel analyze PDF files?
A. UN‑SCAN‑IT gel does not currently analyze PDF files. PDF files often contain multiple pages, imbedded text, and variable dpi settings, which can present difficulties in extracting accurate and useful digitized data. The PDF file should be converted to a standard image format (TIFF, JPG, BMP, PNG, GIF, etc.).

Q. How does UN‑SCAN‑IT gel compare to gel densitometer systems?
A. UN‑SCAN‑IT gel users have compared the results they have obtained with UN‑SCAN‑IT gel to their expensive gel densitometers and have indicated that the UN‑SCAN‑IT gel results compare very favorably to those from the densitometer. In addition, many users have expressed that the UN‑SCAN‑IT gel system is much more intuitive and easy-to-use than other more expensive systems.

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